Objective: Despite advanced treatment options available, drug resistance develops in breast cancer (BC) patients
requiring novel effective drugs. Stylissa carteri, a marine sponge predominantly living in Indonesia territories, has
not been extensively studied as anti-cancer. Therefore, this study targeted to assess the anti-tumor activity of the
ethanol extract of S. carteri in BC cells. Methods: S. carteri was collected from Pramuka Island, at Kepulauan Seribu
National Park, Jakarta, Indonesia and extracted using ethanol. Different BC cells including MDA MB 231, MDA
MB 468, SKBR3, HCC-1954 and MCF-7 cells were treated with this extract for cytotoxic analysis using MTT assay.
Spheroid growth assay and apoptosis assay were conducted in HCC-1954 cells. In addition, cell migration analysis and
synergistic activity with doxorubicin or paclitaxel were conducted in MDA MB 231 cells. This extract was subjected
also for GC-MS analysis. Results: The results show that ethanol extract of S. carteri demonstrated a cytotoxic activity
in BC cells. The IC50 of this extract was lower 15 μg/ml in MDA MB 231, MDA MB 468, SKBR3, and HCC-1954
cells. Moreover, this extract inhibited spheroids growth and induced apoptosis in HCC-1954 cells. It inhibited cell
migration and demonstrated a synergistic activity with doxorubicin or paclitaxel on triggering cell death in MDA MB
231 cells. Furthermore, GC-MS analysis indicated that this extract contained 1,2-Benzenediol, Dibutyl phthalate and
9,12-Octadecadienoic acid, ethyl ester. Conclusion: Our preliminary data indicate a potential anti-tumor activity of
ethanol extract of S. carteri in breast cancer cells.

Identitas Publikasi Ilmiah

Tanggal Publikasi 04 April 2019
Edisi Article 33
Volume 20
Halaman 1199
Tahun 2019
Status publikasi
Relasi Project Penelitian
Staf Pendukung Publikasi Ilmiah
Nama Sebagai

Dokumen Publikasi Ilmiah

Tidak Terdapat Dokumen Untuk Publikasi Ilmiah Bioactive Compounds in the Ethanol Extract of Marine Sponge Stylissa carteri Demonstrates Potential Anti-Cancer Activity in Breast Cancer Cells