AbstractAIM:This study aims to investigate the effect of ATP, EGF and combination of those two to the Natrium Iodide Symporter (NIS) expression in MCF7, SKBR3 and HaCaT cell lines.METHODS:MCF7, SKBR3 and HaCaT cell lines were treated with ATP, EGF and combination of those two for 6, 12 and 24 hours. The expression of NIS mRNA was measured through quantitative-reverse transcription-polymerase chain reaction (qRT-PCR). The NIS protein expression wasconfirmed by immunocytofluorescence.RESULTS:NIS mRNA was expressed in SKBR3 and HaCaT cell lines but not in MCF7. The levels of NIS mRNA expression, after treatment by epidermal growth factor (EGF), adenosine Tri-Phosphate (ATP) or the combination of both for 6 and 12 hours were not significantly different from those of untreated cells. However, the treatment by a combination of ATP and EGF for 24 hours increases the level of NIS mRNA expression by 1.6fold higher than that of the untreated cells (1.6241± 0.3, p<0.05) and protein NIS expression increase significantly by the treatment than untreated cells (P<0.05).CONCLUSION:The level of NIS expression varies among the different subtypes of breast cancer cell lines. MCF7 cell line is representing the luminal A subtype of breast cancer doesnot express NIS. Only SKBR3 cell line express NIS and this subtype might be suitable to receive radioiodine therapy as those cells expressing NIS. A combination treatment of EGF and ATP increases the expression of NIS mRNA and protein at the membrane in SKBR3 cells.

Identitas Publikasi Ilmiah

Tanggal Publikasi 15 Juli 2019
Edisi eISSN
Volume 2
Halaman 7
Tahun 2019
Status publikasi
Relasi Project Penelitian
Staf Pendukung Publikasi Ilmiah
Nama Sebagai

Dokumen Publikasi Ilmiah

Tidak Terdapat Dokumen Untuk Publikasi Ilmiah _______________________________________________________________________________________________________________________________2088https://www.id-press.eu/mjms/indexID Design Press,Skopje, Republic of MacedoniaOpen Access Macedonian Journal of Medical Sciences.2019 Jul 15; 7(13):2088-2092.https://doi.org/10.3889/oamjms.2019.620eISSN: 1857-9655Basic ScienceEpidermal Growth Factor and Adenosine Triphosphate Induce Natrium Iodide Symporter Expression in Breast Cancer Cell Lines